Answer:
Steps in Focusing a specimen using 4 compound microscope
1. Start by rotating the objective lens to lowest power.
2. Place a slide on the stage, label side up, with the coverslip centered
3. On LOW POWER ONLY, use the coarse focus knob to get the object into focus.
4. If you cannot see anything, move the slide slightly while viewing and focusing.
5. If nothing appears, reduce the light and repeat step 4.
6. If you will be viewing fast moving organisms, you may wish to add a drop of thickener such as methyl cellulose or "ProtoSlo" to slow them down by making the fluid more viscous.
7. Slowly lay down the cover slip starting at a 45 degree angle with one edge touching the slide. This helps to prevent air bubbles from forming under the cover slip.
8. Remember that the microscope light is very intense and the organisms will survive longer on the slide if you turn it off when not observing.
