PUT ME IN ORDER A. Pour enough water inside the chamber. B. Close the lid and tighten the screws to ensure an airtight condition, and then switch on electric heater.c. Check for any items left from the previous cycle. D. Once the water inside the chamber boils, allow the air-water mixture to escape through the discharge tube to let all the air inside to be displaced. The complete displacement can be ensured once the water bubbles cease to come out from the pipe E. Adjust the safety valves to maintain the required pressure in the chamber. F. Close the drainage pipe and allow the steam inside is to reach the desired levels (15 lbs. in most cases). G. Open the lid and take the sterilized materials out of the chamber. H. Once the pressure is reached, the whistle blows to remove excess pressure from the chamber. I. Place the materials to be sterilized inside the chamber. J. After the whistle, allow the autoclave to run for a holding period, which is 15 minutes in most cases. K. Open the discharge pipe to allow the entry of air from the outside into the autoclave. L. Switch off the electric heater and allow the autoclave to cool until the pressure gauge indicates the pressure inside has lowered down to that of the atmospheric pressure.​