Directions: Arrange the steps of DNA Replication Process by writing the numbers 1-10 on the space before the

number.

_____1. The separation of the two single strands of DNA creates a ‘Y’ shape called a replication ‘fork’. The two

separated strands will act as templates for making the new strands of DNA.

_____2. A short piece of RNA called a primer produced by an enzyme called primase comes along and binds

to the end of the leading strand. The primer acts as the starting point for DNA synthesis.

_____3. Numerous RNA primers are made by the primase enzyme and bind at various points along the

lagging strand.

_____4. The result of DNA replication is two DNA molecules consisting of one new and one old chain of

nucleotides.

_____5. The double helix structure of the DNA molecule is unzipped. This is carried out by an enzyme called

Helicase.

_____6. DNA polymerase binds to the leading strand and then ‘walks’ along it, adding

new complementary nucleotide bases to the strand of DNA in the 5’ to 3’ direction.

_____7. The new strand is proofread to make sure there are no mistakes in the new DNA sequence.

_____8. Following replication the new DNA automatically winds up into a double helix.

_____9. Finally, an enzyme called DNA ligase seals up the sequence of DNA into two continuous double

strands

_____10. Chunks of DNA, called Okazaki fragments, are then added to the lagging strand also in the 5’ to 3’

direction.​